YOUR BROWSER IS OUT-OF-DATE.

We have detected that you are using an outdated browser. Our service may not work properly for you. We recommend upgrading or switching to another browser.

Sign in

 

Faculty of Chemistry

Marek Orłowski, PhD

Email: marek.orlowski@pwr.edu.pl

Unit: Faculty of Chemistry » Department of Biochemistry, Molecular Biology and Biotechnology

Marek Orłowskiul. Gdańska 7/9, 50-344 Wrocław
building F-4, room C-14
phone +48 71 320 6286

Office hours

  • Monday 13.15-15.15
  • Tuesday 13.15-15.15

Research fields

  • Nuclear receptor superfamily; functional ecdysteroid receptor; 20-hydroxyecdysone receptor (EcR); ultraspiracle protein (Usp); signal transduction pathways; transcription factors; intrinsically disordered proteins (IDPs); intrinsically disordered regions (IDRs); analyses of protein - protein and protein - DNA interactions; overexpression of recombinant proteins in heterologous and native systems; purification of recombinant proteins (platform of AKTA brand chromatography systems, IMAC, gel filtration and more); biochemical and structural analyses of proteins using circular dichroism (CD) spectroscopy and bioinformatics tools; analyses of conformational changes in proteins; analyses of subcellular distribution of proteins using eukaryotic cell lines; analyses of molecular determinants of subcellular distribution of proteins: nuclear localization signals (NLSs), nuclear export signals (NESs) and nucleolus localization signals (NoLSs); fluorescence and confocal microscopy.

Recent papers

2018

  • Orłowski M., Popławska K., Pieprzyk J., Szczygieł-Sommer A., Więch A., Zarębski M., Tarczewska A., Dobrucki J. & Ożyhar A. 2018, Molecular determinants of Drosophila immunophilin FKBP39 nuclear localization, Biological Chemistry, DOI: https://doi.org/10.1515/hsz-2017-0251.

2017

  • Kozłowska M., Tarczewska A., Jakób M., Bystranowska D., Taube M., Kozak M., Czarnocki-Cieciura M., Dziembowski A., Orłowski M., Tkocz K. & Ożyhar, A. 2017, Nucleoplasmin-like domain of FKBP39 from Drosophila melanogaster forms a tetramer with partly disordered tentacle-like C-terminal segments, Scientific Reports, vol. 7, pp. 40405.

2014

  • Pieprzyk J., Zbela A., Jakób M., Ożyhar A. & Orłowski M. 2014, Homodimerization propensity of the intrinsically disordered N-terminal domain of Ultraspiracle from Aedes aegypti, Biochimica et Biophysica Acta, vol. 1844, no. 6, pp. 1153-1166.

2007

  • Jakób M., Kołodziejczyk R., Orłowski M., Krzywda S., Kowalska A., Dutko-Gwóźdź J., Gwóźdź T., Kochman M., Jaskólski M. & Ożyhar A. 2007, Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain, Nucleic Acids Research, vol. 35, no. 8, pp. 2705-2718.

2004

  • Orłowski M., Szyszka M., Kowalska A., Grad I., Zoglowek A., Rymarczyk G., Dobryszycki P., Krowarsch D., Rastinejad F., Kochman M. & Ożyhar A. 2004, Plasticity of the ecdysone receptor DNA binding domain, Molecular Endocrinology, vol. 18, no. 9, pp. 2166-2184.

Papers in DONA database

Politechnika Wrocławska © 2024